The probe of the ultrasonic cell crusher cannot touch the wall and bottom of the container, and the end of the probe is 10-15mm from the liquid level. When using the ultrasonic cell crusher microprobe, the amplitude conditioning should not exceed 70%, otherwise probe damage will be formed.
The container for holding the sample shall be glass container, not other containers; The sample must be aqueous and not inorganic.
Prepare the ultrasonic cell shredder with fluctuations in function, and then throw the plant tissue, plant tissue, bacterial or viral cell aqueous solution that needs to be broken into a beaker or into a beaker, fix the scaffold, fix the ultrasonic cell shredder on the scaffold, and then open the ultrasonic initiator, set the task time, pulse work or continuous work. Set the working power or working amplitude, the ultrasonic cell crusher will be turned on to stop the cell breaking, according to the detailed requirements, after a period of disposal can be broken. When that's done, the cell contents break through the walls and into alcohol or water. Then it can be applied or other superstitious research.
Ultrasonic cell shredder is an ultrasonic experimental instrument for cell fragmentation, in order to extend the service life of ultrasonic cell shredder, the use of ultrasonic cell shredder should pay attention to the following matters.
1, be sure to insert the ultrasonic probe (ultrasonic amplitude transformer) into the sample before starting, do not empty load.
2, ultrasonic horn (ultrasonic probe) into the water depth should be about 1.5cm, the liquid level height above 30mm, the probe should be placed in the center, not attached to the wall. Ultrasonic wave is vertical longitudinal wave, inserted too deep is not easy to form convection, affecting the crushing efficiency.
3. Ultrasonic parameter setting
The working parameters of the ultrasonic cell crusher should be set according to the requirements of the instructions, the more sensitive samples are generally used outside the ice bath, the actual temperature is certainly lower than 25 degrees, and the protein nucleic acid is certainly not denatured.
1) Working hours. The continuous working time of ultrasound should not exceed 5 seconds each time, and the working interval time should be greater than or equal to the ultrasonic time in order to facilitate heat dissipation. The time setting should be based on the principle of short ultrasonic time and multiple ultrasonic times, which can extend the life of ultrasonic cell shredder and ultrasonic probe.
2) Container selection. The container should be selected according to the sample size, and it is not appropriate to choose a large beaker, such as a 20ml sample should be selected 20ml beaker. This can be conducive to the sample in ultrasonic convection, improve the efficiency of ultrasonic crushing. If a 1.5ml EP tube is used to hold the sample in the experiment, the EP tube must be fixed to prevent the liquid level from falling after the ice bath melts and causing no load.
3) Ultrasonic power. It should not be too large, so as not to splash or foam the sample, such as less than 10ml sample capacity, the power should be within 200w, choose 2mm ultrasonic probe, and hit the horn selection switch behind the panel to the corresponding stop; The power of the 10-200ml sample capacity is 200-400w, the 6mm ultrasonic probe is selected, and the amplitude lever behind the panel is hit to the corresponding gear; The sample capacity of more than 200ml is between 300-600w, 10mm ultrasonic probe is selected, and the amplitude lever behind the panel is hit to the corresponding gear; (2MM small probe power is strictly prohibited to exceed 350W)
The above is the ultrasonic cell crusher operation precautions, after the completion of the ultrasonic cell crusher work, should also pay attention to cleaning and maintenance, alcohol can be used to scrub the probe or clean with water.
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